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dc.rights.licenseReconocimiento-NoComercial-CompartirIgual 4.0 Internacional. (CC BY-NC-SA)-
dc.contributor.authorEcheverry, Carolinaes
dc.contributor.authorRicheri, Analíaes
dc.contributor.authorFagetti, Jimenaes
dc.contributor.authorMartınez, Gaby F.es
dc.contributor.authorVignolo, Federicoes
dc.contributor.authorPrunell, Gisellees
dc.contributor.authorCunetti, Leticiaes
dc.contributor.authorMartınez Busi, Marcelaes
dc.contributor.authorPerez, Sandraes
dc.contributor.authorSanchez de Medina, Veronicaes
dc.contributor.authorFerreiro, Carloses
dc.contributor.authorScorza, Ceciliaes
dc.date.accessioned2023-10-31T23:54:00Z-
dc.date.available2023-10-31T23:54:00Z-
dc.date.issued2023-05-22-
dc.identifier.urihttps://hdl.handle.net/20.500.12381/3313-
dc.description.abstractPreclinical research supports the benefits of pharmaceutical cannabis-based extracts for treating different medical conditions (e.g., epilepsy); however, their neuroprotective potential has not been widely investigated. Using primary cultures of cerebellar granule cells, we evaluated the neuroprotective activity of Epifractan (EPI), a cannabis-based medicinal extract containing a high level of cannabidiol (CBD), components like terpenoids and flavonoids, and trace levels of Δ9-tetrahydrocannabinol and the acid form of CBD. We determined the ability of EPI to counteract the rotenone-induced neurotoxicity by analyzing cell viability and morphology of neurons and astrocytes by immunocytochemical assays. The effect of EPI was compared with XALEX, a plant-derived and highly purified CBD formulation (XAL), and pure CBD crystals (CBD). The results revealed that EPI induced a significant reduction in the rotenone induced neurotoxicity in a wide range of concentrations without causing neurotoxicity per se. EPI showed a similar effect to XAL suggesting that no additive or synergistic interactions between individual substances present in EPI occurred. In contrast, CBD did show a different profile to EPI and XAL since a neurotoxic effect per se was observed at the higher concentrations assayed. Medium-chain triglyceride (MCT) oil used in EPI formulation could explain this difference. Our data support a neuroprotective effect of EPI which may provide neuroprotection in different neurodegenerative processes. The results highlight the role of CBD as the active component of EPI but also support the need for an appropriate formulation to dilute pharmaceutical cannabis-based products, which could be critical to avoid neurotoxicity at very high doses.es
dc.description.sponsorshipAgencia Nacional de Investigación e Innovaciónes
dc.language.isoenges
dc.publisherMary Ann Liebert, Inc.es
dc.rightsAcceso abierto*
dc.sourceCannabis and Cannabinoid Researches
dc.subjectCannabis sativaes
dc.subjectEpifractanes
dc.subjectXALEXes
dc.subjectCannabidioles
dc.titleNeuroprotective effect of a pharmaceutical extract of cannabis with high content on CBD against rotenone in primary cerebellar granule cell cultures and the relevance of formulationses
dc.typeArtículoes
dc.subject.aniiCiencias Médicas y de la Salud-
dc.subject.aniiMedicina Básica-
dc.subject.aniiNeurociencias-
dc.identifier.aniiFCE_3_2020_1_162440es
dc.type.versionAceptadoes
dc.identifier.doi10.1089/can.2022.0289-
dc.anii.institucionresponsableInstituto de Investigaciones Biológicas Clemente Establees
dc.anii.institucionresponsablePhytoplant researches
dc.anii.subjectcompleto//Ciencias Médicas y de la Salud/Medicina Básica/Neurocienciases
Aparece en las colecciones: Instituto de Investigaciones Biológicas Clemente Estable

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