Título : Unraveling corynebacterial divisome composition by proximity labeling in the living cell
Autor(es) : Rodriguez, Azalia
Martínez, Mariano
Megrian, Daniela
Rossello, Jessica
Gaday, Quentin
Petit, Julienne
Portela, Maria Magdalena
Ben Assaya, Mathilde
Alzari, Pedro M.
Wehenkel, Anne Marie
Durán, Rosario
Fecha de publicación : 10-nov-2023
Tipo de publicación: Documento de conferencia
Versión: Publicado
Publicado en: XI Reunión de la Sociedad Latinoamericana de Tuberculosis y otras Micobacteriosis, Bucaramanga, Colombia, Noviembre 2023
Areas del conocimiento : Ciencias Naturales y Exactas
Ciencias Biológicas
Bioquímica y Biología Molecular
Otros descriptores : Interactomics
Cell division
Resumen : Bacterial cell division is directed by the divisome, a protein complex whose assembly begins with the polymerization of FtsZ at midcell to form a ring (Z-ring) This structure participates in the recruitment of other divisome proteins, that in the case of the model bacilli (Escherichia coli and Bacillus subtilis) have been identified and characterized. However, the order Corynebacteriales (that includes important human pathogens as Mycobacterium tuberculosis and Corynebacterium diphtheriae) lacks recognizable homologues for many of these cell division proteins, and the ones fulfilling these missing functions are yet to be identified. To identify the unknown pieces of the corynebacterial divisome, we developed and optimized a proteomic strategy based on proximity biotinylation in the living cell, using Corynebacterium glutamicum as a model organism. We generated a strain expressing FtsZ fused to an engineered ascorbate peroxidase (APEX2). APEX2 catalyzes the oxidation of phenol biotin in the presence of H2O2 giving rise to a radical that reacts with amino acids of nearby proteins. This allowed us to label the proteomic environment of FtsZ in the living cell, and its purification and identification by Mass Spectrometry. We corroborated that APEX2 is active in the biochemical background of C. glutamicum, and optimized the labelling strategy to guarantee the identification of physiologically relevant FtsZ neighbours. We identified a confident list of 253 FtsZ neighbors, that includes known cell division proteins as well as an important number of non-characterized proteins, which represents putative new divisome components. We focused on hypothetical membrane proteins, that might mediate membrane anchor of the Z-ring, as most of the proteins fulfilling this role in E. coli and B. subtilis are not present in corynebacterial genomes. We generate strains expressing the selected candidates fused to a fluorescent proof to evaluate their subcellular localization and their interaction with FtsZ. The results allowed us to identify new conserved membrane bound components of the corynebacterial divisome. Their precise role in cell division, the molecular details of its interaction with the Z-ring and its regulation by protein phosphorylation are being studied.
URI / Handle: https://hdl.handle.net/20.500.12381/3335
Institución responsable del proyecto: Institut Pasteur de Montevideo
Instituto de Investigaciones Biológicas Clemente Estable
Financiadores: Agencia Nacinal de Investigación e Innovación
ECOS-Sud France-Uruguay U20B02
Identificador ANII: FCE_1_2019_1_155569
Nivel de Acceso: Acceso abierto
Licencia CC: Reconocimiento-NoComercial-CompartirIgual 4.0 Internacional. (CC BY-NC-SA)
Aparece en las colecciones: Institut Pasteur de Montevideo

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