Título : Genotyping and Multivariate Regression Trees Reveal Ecological Diversification within the Microcystis aeruginosa Complex along a Wide Environmental Gradient
Autor(es) : Martínez de la Escalera, Gabriela
Segura, Angel
Kruk, Carla
Ghattas, Badih
Cohan, Frederick
Iriarte, Andrés
Piccini, Claudia
Fecha de publicación : 8-feb-2022
Tipo de publicación: Artículo
Versión: Publicado
Publicado por: American Society for Microbiology
Publicado en: Applied and Environmental Microbiology
Areas del conocimiento : Ciencias Naturales y Exactas
Ciencias Biológicas
Matemática Aplicada
Biología Celular, Microbiología
Otros descriptores : Ecotypes
Multivariate CART
Microcystis aeruginosa complex
Resumen : Addressing the ecological and evolutionary processes underlying biodi- versity patterns is essential to identify the mechanisms shaping community structure and function. In bacteria, the formation of new ecologically distinct populations (eco- types) is proposed as one of the main drivers of diversification. New ecotypes arise when mutations in key functional genes or acquisition of new metabolic pathways by horizontal gene transfer allow the population to exploit new resources, permit- ting their coexistence with the parental population. We previously reported the pres- ence of microcystin-producing organisms of the Microcystis aeruginosa complex (toxic MAC) through an 800-km environmental gradient ranging from freshwater to estuarine-marine waters in South America. We hypothesize that the success of toxic MAC in such a gradient is due to the existence of very closely related populations that are ecologically distinct (ecotypes), each specialized to a specific arrangement of environmental variables. Here, we analyzed toxic MAC genetic diversity through quantitative PCR (qPCR) and high-resolution melting analysis (HRMA) of a functional gene (mcyJ, microcystin synthetase cluster). We explored the variability of the mcyJ gene along the environmental gradient by multivariate classification and regression trees (mCART). Six groups of mcyJ genotypes were distinguished and associated with different combinations of water temperature, conductivity, and turbidity. We propose that each mcyJ variant associated with a defined environmental condition is an eco- type (or species) whose relative abundances vary according to their fitness in the local environment. This mechanism would explain the success of toxic MAC in such a wide array of environmental conditions.
URI / Handle: https://hdl.handle.net/20.500.12381/3485
DOI: https://journals.asm.org/doi/full/10.1128/aem.01475-21#:~:text=https%3A//doi.org/10.1128/aem.01475%2D21
Institución responsable del proyecto: Instituto de Investigaciones Biológicas Clemente Estable
Universidad de la República. Centro Universitario Regional del Este
Financiadores: Agencia Nacional de Investigación e Innovación
Identificador ANII: FCE_1_2019_1_156308
Nivel de Acceso: Acceso abierto
Licencia CC: Reconocimiento 4.0 Internacional. (CC BY)
Aparece en las colecciones: Instituto de Investigaciones Biológicas Clemente Estable

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