Exploring Elastin-Like Polypeptide Tags and Mini-intein for Recombinant Protein Purification in Leishmania tarentolae

Abstract

Recombinant technology has enabled the production of a wide range of proteins with applications in various fields, including reproduction, food supplements, and medicine. However, the high costs associated with producing and purifying recombinant proteins limit their widespread use in the veterinary field. Therefore, the development of alternative, low-cost strategies for production and purification is essential to address these challenges. The non-pathogenic eukaryotic protozoan Leishmania tarentolae has emerged as an affordable expression system for heterologous proteins, providing glycosylation profiles similar to those of higher eukaryotes. Despite this, the cost of recombinant protein purification remains a significant barrier to expanding the use of recombinant products in areas such as livestock. In this study, we evaluate an unconventional purification tag, the elastin-like polypeptide (ELP), for purifying recombinant proteins secreted by L. tarentolae. We also explored the use of the mini-intein ΔI-CM as a cleavable linker between ELP and the target protein. This intein was found to undergo intracellular self-cleavage, precluding its use in secretion-based applications in this host. Despite this limitation, the ELP-mediated purification from the culture supernatant was successfully achieved with a high purity using 0.6 M ammonium sulfate at 25-30 °C. To our knowledge, this is the first report of ELP-based protein purification in a protozoan host, representing a promising tool with broad potential for applications in molecular biology, pharmaceutical development, and other fields that require high-purity proteins with an improved cost-to-benefit ratio.